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SRX1526338: GSM2035723: FL-HSPC LKO_infected experiment_2 (LKO in shHOXA7gene_exp.diff); Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 14.9M spots, 761.1M bases, 617.8Mb downloads

Submitted by: NCBI (GEO)
Study: RNA-seq expression data from FL-HSPCs after HOXA7 knockdown
PRJNA308425 • SRP068278 • All experiments • All runs
show Abstracthide Abstract
HOXA7 regulates FL-HSPC self-renewal in vitro and in vivo. We profiled FL-HSPCs after HOXA7 knockdown, to assess the gene expression programs regulated by HOXA7. Overall design: CD34+CD38-CD43+CD90+ HSPCs were infected with lentiviral shRNA vector targeting HOXA7 (sh-HOXA7). The emtpy lentiviral vector (LKO) was used as a control. Cells were expanded on op9 for 5 days and selected for puromicin resistance for 3 days, than sorted for HSPC immunophenotype.
Sample: FL-HSPC LKO_infected experiment_2 (LKO in shHOXA7gene_exp.diff)
SAMN04395230 • SRS1244229 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: RNA was purified from sorted cells using RNeasy Mini Kit (QIAGEN), according to manufacturer's instructions. Ovation Rna-seq system System v2 (Nugen), followed by KAPA LTP Library Preparation Kit
Experiment attributes:
GEO Accession: GSM2035723
Links:
Runs: 1 run, 14.9M spots, 761.1M bases, 617.8Mb
Run# of Spots# of BasesSizePublished
SRR309599014,924,155761.1M617.8Mb2016-05-17

ID:
2157609

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